Journal: Scientific Reports

Article Title: High accumulation of Mx2 renders limited multiplication of oncolytic herpes simplex virus-1 in human tumor cells

doi: 10.1038/s41598-021-00691-y

Figure Lengend Snippet: Cell lines used in this study.

Article Snippet: SCC9 , Human tongue squamous carcinoma cell , DMEM + 10%FBS , ATCC.

Techniques:

Journal: Journal of Cellular and Molecular Medicine

Article Title: 7‐Epitaxol induces apoptosis in cisplatin‐resistant head and neck squamous cell carcinoma via suppression of AKT and MAPK signalling

doi: 10.1111/jcmm.17602

Figure Lengend Snippet: 7‐ Epitaxol induces cell cycle arrest and inhibits cell proliferation in Cis‐SCC9 and Cis‐SAS cells. (A, B) Cis‐SCC‐9 and Cis‐SAS were analysed by colony formation assay, that cells cultured in condition medium presence of 7‐E (0–100 nM) for 14 days. (C) After treated 7‐E (0–100 nM) for 24 h. Cells were used PI stained and flow cytometry performed to estimate cell cycle phase distribution. (D) Quantification of different cell cycle phase of sub‐G1, G0/G1, S and G2/M. Data are presented as mean ± SD ( n = 3). * p < 0.05, compared with the control group.

Article Snippet: Two HNSCC cell lines including SCC‐9 and SAS (Japanese Collection of Research Bioresources Cell Bank, JCRB Cell Bank) were cultured in Dulbecco's Modified Eagle Medium‐F12 (DMEM; Life Technologies, Grand Island, NY) supplemented with 10% foetal bovine serum, 0.1 mM nonessential amino acids, 1 mM glutamine, 1% penicillin/streptomycin (10,000 U/ml penicillin and 10 mg/ml streptomycin), 1.5 g/L sodium bicarbonate and 1 mM sodium pyruvate.

Techniques: Colony Assay, Cell Culture, Staining, Flow Cytometry, Control

Journal: Journal of Cellular and Molecular Medicine

Article Title: 7‐Epitaxol induces apoptosis in cisplatin‐resistant head and neck squamous cell carcinoma via suppression of AKT and MAPK signalling

doi: 10.1111/jcmm.17602

Figure Lengend Snippet: 7‐ Epitaxol induces apoptosis in Cis‐SCC9 and Cis‐SAS cells. After treated 7‐E (0–100 nM) for 24 h. (A, B) Used DAPI stain assay to determine DNA condensation with fluorescence microscopy. (C) Cell were stained Annexin V/PI and used flow cytometry to revealed 7‐E induced apoptosis. (D) Quantitative relative the percentage of apoptosis cells (including early and late state). Data are presented as mean ± SD ( n = 3). * p < 0.05, compared with the control group.

Article Snippet: Two HNSCC cell lines including SCC‐9 and SAS (Japanese Collection of Research Bioresources Cell Bank, JCRB Cell Bank) were cultured in Dulbecco's Modified Eagle Medium‐F12 (DMEM; Life Technologies, Grand Island, NY) supplemented with 10% foetal bovine serum, 0.1 mM nonessential amino acids, 1 mM glutamine, 1% penicillin/streptomycin (10,000 U/ml penicillin and 10 mg/ml streptomycin), 1.5 g/L sodium bicarbonate and 1 mM sodium pyruvate.

Techniques: Staining, Fluorescence, Microscopy, Flow Cytometry, Control

Journal: Journal of Cellular and Molecular Medicine

Article Title: 7‐Epitaxol induces apoptosis in cisplatin‐resistant head and neck squamous cell carcinoma via suppression of AKT and MAPK signalling

doi: 10.1111/jcmm.17602

Figure Lengend Snippet: Intrinsic pathway and the extrinsic pathway were regulated by 7‐Epitaxol in Cis‐SCC9 and Cis‐SAS cells. After treated 7‐E (0–100 nM) for 24 h. (A) Mitochondrial membrane potential measurement assay was used by flow cytometry. (B) The data was analysed by Muse Cell Analyzer (Millipore). (C) Analyse the expression of extrinsic pathway control proteins, including Fas, TNF‐R1, DR5, DcR3, DcR2 and beta‐actin by Western blot. (D) Quantitative relative density of each protein levels was normalized to beta‐actin. Data are presented as mean ± SD ( n = 3). * p < 0.05, compared with the control group.

Article Snippet: Two HNSCC cell lines including SCC‐9 and SAS (Japanese Collection of Research Bioresources Cell Bank, JCRB Cell Bank) were cultured in Dulbecco's Modified Eagle Medium‐F12 (DMEM; Life Technologies, Grand Island, NY) supplemented with 10% foetal bovine serum, 0.1 mM nonessential amino acids, 1 mM glutamine, 1% penicillin/streptomycin (10,000 U/ml penicillin and 10 mg/ml streptomycin), 1.5 g/L sodium bicarbonate and 1 mM sodium pyruvate.

Techniques: Membrane, Flow Cytometry, Expressing, Control, Western Blot

Journal: Journal of Cellular and Molecular Medicine

Article Title: 7‐Epitaxol induces apoptosis in cisplatin‐resistant head and neck squamous cell carcinoma via suppression of AKT and MAPK signalling

doi: 10.1111/jcmm.17602

Figure Lengend Snippet: 7‐ Epitaxol activates caspase pathway and regulated Bcl‐2 family in Cis‐SCC9 and Cis‐SAS cells. Western blotting was used to measure the expression of regulated proteins after 24 h of 7‐E treatment in (A, B) the caspase pathway related proteins and (C, D) the Bcl‐2 family related proteins. (E, F) Western blotting was used to measure the expression of caspase pathway related proteins after 24 h of Z‐VAD‐FMK (Z‐VAD) (20 μM) combined with 7‐E in Cis‐SCC9 and Cis‐SAS cells. (G) Cell viability was measured by MTT assay. Quantitative relative density of each protein levels was normalized to beta‐actin. Data are presented as mean ± SD ( n = 3). * p < 0.05, compared with the control group.

Article Snippet: Two HNSCC cell lines including SCC‐9 and SAS (Japanese Collection of Research Bioresources Cell Bank, JCRB Cell Bank) were cultured in Dulbecco's Modified Eagle Medium‐F12 (DMEM; Life Technologies, Grand Island, NY) supplemented with 10% foetal bovine serum, 0.1 mM nonessential amino acids, 1 mM glutamine, 1% penicillin/streptomycin (10,000 U/ml penicillin and 10 mg/ml streptomycin), 1.5 g/L sodium bicarbonate and 1 mM sodium pyruvate.

Techniques: Western Blot, Expressing, MTT Assay, Control

Journal: Journal of Cellular and Molecular Medicine

Article Title: 7‐Epitaxol induces apoptosis in cisplatin‐resistant head and neck squamous cell carcinoma via suppression of AKT and MAPK signalling

doi: 10.1111/jcmm.17602

Figure Lengend Snippet: 7‐Epitaxol induces apoptosis by affecting the AKT, ERK1/2 and p38 MAPK pathway in Cis‐SCC9 and Cis‐SAS cells. Cell lines were pre‐treatment with specific inhibitors (U0126, LY294002, SB203580, SP600125) or without for 1 h, then treated 7‐E for 24 h. Western blotting was used to measure the expression of regulated proteins (A, B) the AKT and MAPK pathway, (C‐F) the caspase pathway proteins. Quantitative relative density of each protein levels was normalized to beta‐actin. Data are presented as mean ± SD ( n = 3). * p < 0.05, compared with the control group. # p < 0.05, compared with the cells treated with 7‐E (100 nM).

Article Snippet: Two HNSCC cell lines including SCC‐9 and SAS (Japanese Collection of Research Bioresources Cell Bank, JCRB Cell Bank) were cultured in Dulbecco's Modified Eagle Medium‐F12 (DMEM; Life Technologies, Grand Island, NY) supplemented with 10% foetal bovine serum, 0.1 mM nonessential amino acids, 1 mM glutamine, 1% penicillin/streptomycin (10,000 U/ml penicillin and 10 mg/ml streptomycin), 1.5 g/L sodium bicarbonate and 1 mM sodium pyruvate.

Techniques: Western Blot, Expressing, Control